Grauzone and completion of meiosis during drosophila oogenesis by Bin Chen

Cover of: Grauzone and completion of meiosis during drosophila oogenesis | Bin Chen

Published by Kluwer Academic Publishers in Boston .

Written in English

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Subjects:

  • Zinc-finger proteins,
  • Meiosis,
  • Drosophila,
  • Oogenesis,
  • Meiosis -- genetics,
  • Drosophila -- cytology,
  • Oogenesis -- physiology,
  • Zinc Fingers -- genetics

Edition Notes

Includes bibliographical references (p. [69]-76) and index.

Book details

Statementby Bin Chen.
Classifications
LC ClassificationsQP552.Z55 C474 2001, QP552.Z55 C474 2001
The Physical Object
Paginationxx, 80 p. :
Number of Pages80
ID Numbers
Open LibraryOL18160275M
ISBN 100792373634
LC Control Number2001029437

Download Grauzone and completion of meiosis during drosophila oogenesis

Grauzone and Completion of Meiosis During Drosophila Oogenesis describes the work behind a major, award winning discovery: the establishment of a new pathway that specifically regulates the female meiosis, a process essential for sexual reproduction.

This book chronicles a new gene mapping method and the cloning and documentation of several types of genes that were proven to have significant influence on the cell : Bin Chen. Grauzone and Completion of Meiosis During Drosophila Oogenesis describes the work behind a major, award winning discovery: the establishment of a new pathway that specifically regulates the female meiosis, a process essential for sexual reproduction.

These results demonstrate that activation of cortex transcription by grauzone is necessary for the completion of meiosis in Drosophila oocytes, and establish a new pathway that specifically regulates the female meiotic cell by: function and Grauzone protein binds to the cortex promoter.

These results demonstrate that activation of cortextranscription by is necessary for thegrauzone completion of meiosis in Drosophilaoocytes, and establish a new pathway that specifically regulates the female meiotic cell cycle. Key words: grauzone, cortex, Meiosis, Drosophila, Oocyte SUMMARYCited by: Summary: Mutations in cortex and grauzone cause abnormal arrest in Drosophila female was mapped to a 14 kb interval in 26F–27A by the male recombination mapping method.

While these experiments mapped the gene accurately, they also illustrated some complexities of this method. Rescue results showed that a kb genomic fragment from this interval was able to fully Cited by: The grauzone and cortex genes are required for the completion of meiosis in Drosophila oocytes.

The grauzone gene encodes a C2H2-type zinc-finger transcription factor that binds to the cortex. Science: Life Sciences Human Anatomy Physiology: Oogenesis Books - Save now on titles like The Oogenesis And Early Embryology Of Ascaris Oanis Werner (), Grauzone and Completion of Meiosis During Drosophila Oogenesis, and other Science: Life Sciences Human Anatomy Physiology: Oogenesis Books.

In book: Drosophila Oogenesis: Methods and Protocols (pp) genetic change during oogenesis is the recently characterized. resumption and completion of meiosis [ 53]. Oogenesis: The Storage Of Development Information by Raven Chr.

P and a great selection of related books, art and collectibles available now at Drosophila oogenesis was initially studied for its role in patterning the embryo, but it has since become a powerful model system for investigating many aspects of cell and developmental biology. Its usefulness can be attributed partly to the genetic tractability of Drosophila as a model system; however, it is also one of the most intensively and successfully studied stages in the development.

Gametogenesis (Spermatogenesis and Oogenesis) Gametogenesis, the production of sperm and eggs, takes place through the process of meiosis. During meiosis, two cell divisions separate the paired chromosomes in the nucleus and then separate the chromatids that were made during an earlier stage of the cell’s life cycle, resulting in gametes that each contain half the number.

In: Grauzone and Completion of Meiosis During Drosophila Oogenesis. Springer, Boston, MA. DOI ; Publisher Name Springer, Boston, MA; Print ISBN ; Online ISBN ; eBook Packages Springer Book Archive; Buy this book on publisher's site. Meiosis, Spermatogenesis, Oogenesis. STUDY.

PLAY. Terms in this set () sexual reproduction. union of sex cells or gametes (sperm, oocyte) development. formation of an adult from a single-celled embryo or zygote. genes. end of meiosis 1, each chromosome still has 2 chromatids, so now needs to reduce the amount of DNA back to normal (split.

During oogenesis of many insects, including Drosophila, cytoplasmic bridges called ring canals allow the flow of materials among the nurse cells and the oocyte. Ring canals can be visualized by staining with fluorescently labeled phalloidin, which binds to filamentous Actin, or with antibodies directed against ring canal components.

This transgene rescued the meiosis arrest of embryos from these mutants and allowed their complete development, indicating that activation of cortex transcription is the primary role of Grauzone during Drosophila oogenesis.

These experiments further define a new transcriptional pathway that controls the meiotic cell cycle in Drosophila oocytes. The grauzone and cortex genes are required for the completion of meiosis in Drosophila oocytes. The grauzone gene encodes a C2H2-type zinc-finger transcription factor that binds to the cortex promoter and is necessary for high-level activation of cortex transcription.

Drosophila oogenesis Rebecca Bastock and Daniel St Johnston Drosophila oogenesis was initially studied for its role in patterning the embryo, but it has since become a powerful model system for investigating many aspects of cell and developmental biology.

Its usefulness can be attributed partly to the genetic tractability of Drosophila. The grauzone and cortex genes are required for the completion of meiosis in Drosophila oocytes. The grauzone gene encodes a C2H2-type zinc-finger transcription factor that binds to the cortex promoter and is necessary for high-level activation of cortex transcription.

Here we define the region of the cortex promoter to which Grauzone binds and show that the binding occurs through the C-terminal, zinc. The grauzone and cortex genes are required for the completion of meiosis in Drosophila oocytes. The grauzone gene encodes a C2H2-type zinc-finger transcription factor that binds to the cortex promoter and is necessary for high-level activation of cortex transcription.

Here we define the region of the cortex promoter to which Grauzone binds and show that the binding occurs through the C-terminal, zinc. D.L. Pittman and J.C. Schimenti, Recombination in the Mammalian Germline.W. Wahls, Hot Spots of Recombination.B.D. McKee, Pairing Sites and the Role of Chromosomes Pairing in Meiosis and Spermatogenesis in Male Drosophila.

Zolan, Role of DNA Repair Genes in Meiosis.E.M. Eddy and D.A. OBrien, Gene Expression During Mammalian Meiosis.T. Ashley and A. Plug, Caught in the Act:. Key meiotic events in many organisms are controlled at the translational level. In this study, we examine the role of translational regulation in the meiotic cell cycle order to address this question, we developed a system for activatingDrosophilaoocytesin this method, hundreds of mature oocytes can be activated to resume and complete meiosis.

Gametogenesis occurs when a haploid cell (n) is formed from a diploid cell (2n) through meiosis. We call gametogenesis in the male spermatogenesis and it produces spermatozoa. In the female, we call it oogenesis. It results in the formation of ova.

This article covers both oogenesis and spermatogenesis. Huynh, J. and St. Johnston, D. () The role of BicD, egl, orb and the microtubules in the restriction of meiosis to the Drosophila oocyte. DevelopmentGhabrial, A.

and Schupbach, T. () Activation of a meiotic checkpoint regulates translation of Gurken during Drosophila oogenesis. Nature Cell Biol. 1, Major stages of oogenesis What follows is a description of the major oogenic events using primarily examples from the fruit fly, Drosophila melanogaster.

This provides an example of a polytrophic ovary in which the nurse cells are derived from the same germ cell lineage as the oocyte. The first step m oogenesis occurs when an.

Description: Grauzone and Completion of Meiosis During Drosophila Oogenesis describes the work behind a major, award winning discovery: the establishment of a new pathway that specifically regulates the female meiosis, a process essential for sexual reproduction.

This book chronicles a new gene mapping method and the cloning and documentation. A second major reason that Drosophila ovaries are utilized as a model in which to study meiosis is the elegant, ordered simplicity by which oocyte development (oogenesis) proceeds.

The Drosophila ovary consists of 15–20 ovarioles, each composed of progressively more developed follicles (Spradling ; Figure 2, A–C).Each follicle will not only harbor the oocyte, but also provide a. Female meiosis takes place in the context of oogenesis.

Drosophila females have two ovaries, each containing approximately 18 ovarioles, chains of progressively developing egg chambers, each consisting of an oocyte and associated support cells. Ovarioles are organized from anterior to posterior.

The anterior contains the germarium, the location of germline stem cells and somatic stem. Proteome Remodeling During Drosophila Oocyte Maturation. Oocyte maturation is a late event of oogenesis.

Drosophila oogenesis unfolds through 14 morphologically distinct stages that can be recovered by dissection (Fig. 1A) ().Recovered egg chambers are composed of three cell types: Germ-line–derived oocyte and nurse cells and somatic follicle cells.

These results suggest that cpb-1 regulates the translation of one or more mRNAs that are critical for completion of meiosis and perhaps other processes during spermatogenesis (Luitjens et al., ). The ife-1 gene encodes an eIF4E mRNA cap binding protein that is associated with P granules during germline proliferation.

To address the role of ATM in meiosis, we undertook an analysis of DSB formation and repair during Drosophila oogenesis. This work was made possible by a temperature-sensitive allele of tefu (tefu 8; Silva et al., ; Pedersen et al., ), allowing us to bypass the pupal lethality associated with tefu-null mutants.

Our findings suggest that. Oogenesis can take a very long time: from several days in Drosophila, to several months or even years in amphibians such as Xenopus and Rana, to several decades in humans.

Maternal transcripts are posttranscriptionally regulated from the moment of their synthesis, during oogenesis, often because they are not used for an extensive period after.

Meiosis (/ m aɪ ˈ oʊ s ɪ s / (); from Greek μείωσις, meiosis, meaning "lessening") is a special type of cell division of germ cells in sexually-reproducing organisms used to produce the gametes, such as sperm or egg involves two rounds of division that ultimately result in four cells with only one copy of each paternal and maternal chromosome ().

As with mitosis, DNA replication occurs prior to meiosis during the S-phase of the cell cycle. Meiosis is a series of events that arrange and separate chromosomes and chromatids into daughter cells. During the interphases of meiosis, each chromosome is duplicated.

In meiosis, there are two rounds of nuclear division resulting in four nuclei and. We observed 4 forms of Cyclin A present during oogenesis: 2 fast migrating, premeiotic forms, 4 forms in prometaphase I, the slowest migrating form in metaphase I, and all 4 forms after the completion of meiosis (Fig.

1 A). These 4 forms match the forms present in early Drosophila embryos (Fig. 2 A). Mature Oogonia is knownas primary oocytes, which undergoes meiosis, howerer, meiosis stopped at Prophase-I. iii) Meiotic phase: Completion of meiosis-I produces a secondary oocyte and a polar body.

The second meiosis division os Secondary Oocyte occur with unequal distribution of cytoplaswm producing large egg and a small second polar body. Oogenesis is the process of formation of a mature female gamete. It begins during the embryonic development stage of the female. Around a couple of million oogonia.

Abstract. In contrast to the extensive analysis of the regulation of Cyclin B protein levels during developmental progression through meiosis in oogenesis, little is known about Cyclin A. Repression of cyclin A translation early in prophase I in Drosophila is important to maintain the oocyte in meiosis, and this has been shown to be mediated by deadenylation of the mRNA and inhibition by the.

I think your book was just backtracking and saying that during oogenesis at the point of Prophase I crossing over occurs. During oogenesis at Prophase I the homologous chromosomes inside the primary oocyte line up with each other and crossing over occurs between the maternal genetic information found in all cells and the paternal copy of the.

In Drosophila, maternal effect genes are expressed during oogenesis that produce translationally inactive mRNAs are stored regionally in the cytoplasm of the oocyte for future translation during such processes as oocyte maturation, fertilization and early embryonic development.

The cell-division cycle in eukaryotes is a complex process that involves cyclins, cdks and multiple checkpoints that eventually lead to cell division. There are two different types of cell division which are Meiosis and Mitosis.

Meiosis is the type of cell division which involves gametes or sex cells that are involved in sexual reproduction.

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